Shigella flexneri surface protein IcsA is sufficient to direct actin-based motility.
نویسندگان
چکیده
منابع مشابه
Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.
Shigella, the causative agent of bacillary dysentery, is capable of directing its own movement in the cytoplasm of infected epithelial cells. The bacterial surface protein VirG recruits host components mediating actin polymerization, which is thought to serve as the propulsive force. Here, we show that neural Wiskott-Aldrich syndrome protein (N-WASP), which is a critical target for filopodium f...
متن کاملActivation of the Cdc42 Effector N-Wasp by the Shigella flexneri Icsa Protein Promotes Actin Nucleation by Arp2/3 Complex and Bacterial Actin-Based Motility
To propel itself in infected cells, the pathogen Shigella flexneri subverts the Cdc42-controlled machinery responsible for actin assembly during filopodia formation. Using a combination of bacterial motility assays in platelet extracts with Escherichia coli expressing the Shigella IcsA protein and in vitro analysis of reconstituted systems from purified proteins, we show here that the bacterial...
متن کاملN-WASP is an important protein for the actin-based motility of Shigella flexneri in the infected epithelial cells.
quently spread within and between the cells are a prerequisite for causing dysentery. The capacity of the bacteria to spread in the cytoplasm and then move into adjacent epithelial cells is know as intra / intercellular spreading, respectively, and requires the bacterial functions encoded by the virG (icsA) gene (1, 2, 3). After invasion of epithelial cells, Shigella become surrounded by F-acti...
متن کاملIdentification of Shigella flexneri IcsA Residues Affecting Interaction with N-WASP, and Evidence for IcsA-IcsA Co-Operative Interaction
The Shigella flexneri IcsA (VirG) protein is a polarly distributed outer membrane protein that is a fundamental virulence factor which interacts with neural Wiskott-Aldrich syndrome protein (N-WASP). The activated N-WASP then activates the Arp2/3 complex which initiates de novo actin nucleation and polymerisation to form F-actin comet tails and allows bacterial cell-to-cell spreading. In a prev...
متن کاملCell migration
distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility. Mol. (1995). The unrelated surface proteins ActA of Listeria monocytogenes and IcsA of Shigella flexneri are sufficient to confer actin-based motility on Listeria innocua and Escherichia coli respectively. Identification of icsA, a plasmid locus of Shigella flexneri that governs ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.14.6572